Rapid Measurement of Protein Osmotic Second Virial Coefficients by Self-Interaction Chromatography
نویسندگان
چکیده
منابع مشابه
Predictive crystallization of ribonuclease A via rapid screening of osmotic second virial coefficients.
Important progress has been made in recent years toward developing a molecular-level understanding of protein phase behavior in terms of the osmotic second virial coefficient, a thermodynamic parameter that characterizes pairwise protein interactions. Yet there has been little practical application of this knowledge to the field of protein crystallization, largely because of the difficult and t...
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Protein self-interaction is important in protein crystal growth, solubilization, and aggregation, both in vitro and in vivo, as with protein misfolding diseases, such as Alzheimer's. Although second virial coefficient studies can supply invaluable quantitative information, their emergence as a systematic approach to evaluating protein self-interaction has been slowed by the limitations of tradi...
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The effects of ammonium sulphate concentration on the osmotic second virial coefficient (BAA/MA) for equine serum albumin (pH 5.6, 20 degrees C) have been examined by sedimentation equilibrium. After an initial steep decrease with increasing ammonium sulphate concentration, BAA/MA assumes an essentially concentration-independent magnitude of 8-9 ml/g. Such behaviour conforms with the statistica...
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In the present work, a modified correlation is presented for the second virial coefficients of both polar and nonpolar fluids based on the corresponding states principle. The second virial coefficients of gaseous polar and non-polar compounds were calculated and compared with experimental data and with other correlations. Comparisons with the existing correlations show that the present work is ...
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6,123,819 A * 9/2000 Peeters ....................... 204/452 6,361,958 B1 * 3/2002 Shieh et al. ................. 435/7.1 6,524,790 B1 * 2/2003 Kopf-Sill et al. .............. 435/6 6,585,939 B1 7/2003 Dapprich 6,599,436 B1 7/2003 Matzke et al. 6,613,512 B1 9/2003 Kopf-Sill et al. 6,649,358 B1 * 11/2003 Parce et al. .................. 435/7.2 6,699,665 B1 * 3/2004 Kim et al. ......................
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2002
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(02)75513-6